pbio.3002544.s011.pdf (521.82 kB)
Microscale thermophoresis (MST) binding assay of VP35 with Ubiquitin and Compounds.
journal contribution
posted on 2024-02-29, 18:53 authored by Carlos A. Rodríguez-Salazar, Sarah van Tol, Olivier Mailhot, Maria Gonzalez-Orozco, Gabriel T. Galdino, Abbey N. Warren, Natalia Teruel, Padmanava Behera, Kazi Sabrina Afreen, Lihong Zhang, Terry L. Juelich, Jennifer K. Smith, María Inés Zylber, Alexander N. Freiberg, Rafael J. Najmanovich, Maria I. Giraldo, Ricardo RajsbaumTagged WT VP35 in complex different interactors to determine if there are shifts normalized fluorescence (Fnorm) (A) K63-linked Ubiquitin (a mixture of n = 3–12), (B) pCEBS, and (C) SFC. The data underlying the graphs shown in the figure can be found in S1 Data.
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