ppat.1009706.s013.pdf (328.86 kB)
Methods of different assays such as preparation of RBD and Spike Pseudovirus, titre detrmination of Spike pseudovirus and SARS-CoV-2 virus, Cell viability assays, qPCR and western blot techniques, image segmentation methods, synthesis of Niclosamide, extraction of Esomoprazole and Pantoprazole are detailed in S1 Text.
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posted on 2021-07-12, 17:46 authored by Chaitra Prabhakara, Rashmi Godbole, Parijat Sil, Sowmya Jahnavi, Shah-e-Jahan Gulzar, Thomas S. van Zanten, Dhruv Sheth, Neeraja Subhash, Anchal Chandra, Akshatha Shivaraj, Patricia Panikulam, Ibrahim U, Vijay Kumar Nuthakki, Theja Parassini Puthiyapurayil, Riyaz Ahmed, Ashaq Hussain Najar, Sai Manoz Lingamallu, Snigdhadev Das, Bhagyashri Mahajan, Praveen Vemula, Sandip B. Bharate, Parvinder Pal Singh, Ram Vishwakarma, Arjun Guha, Varadharajan Sundaramurthy, Satyajit Mayor(PDF)
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pH-dependent endocytic pathwayuptakeinhibitionNH 4 ClNiclosamideACE 2. Ectopic expressionattenuates Spike-pseudovirus entryEndosomal acidification inhibitorsCLICSARS-CoV -2SARS-CoV -2 spike proteinBafilomycinA 1endosomal pHCGinfectiontarget SARS-CoV -2 entryAGS-ACE 2 cellsSARS-CoV -2 entry inhibitorreceptor binding domainRBDhost endo-lysosomal network
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