posted on 2021-07-02, 16:06authored byHuichang Gao, Jin Xiao, Yingqi Wei, Hui Yang, Fen Zou
The
extracellular matrix microenvironment, including chemical constituents
and topological structure, plays key role in regulating the cell behavior,
such as adhesion, proliferation, differentiation, apoptosis, etc.
Until now, to investigate the relationship between surface texture
and cell response, various ordered patterns have been prepared on
the surface of different matrixes, whereas almost all these strategies
depend on advanced instruments or severe synthesis conditions. Herein,
cell-mediated mineralization method has been applied to construct
nanopattern on the surface of β-TCP scaffold. The formation
process, morphology, and composition of the final pattern were characterized,
and a possible mineralization mechanism has been proposed. Moreover,
the cell behavior on the nanopattern has been investigated, and the
results showed that the mouse bone marrow mesenchyme stem cells (mBMSCs)
display good affinity with the nanopattern, which was manifested by
the good proliferation and osteogenic differentiation status of cells.
The synthetic strategy may shed light to construct advanced topological
structures on other matrixes for bone repair.