pone.0258753.s001.pdf (467.21 kB)
Long PCR analysis of 7.1Anguil strain inversion.
journal contribution
posted on 2021-10-28, 17:37 authored by Ariel Amadio, James L. Bono, Matías Irazoqui, Mariano Larzábal, Wanderson Marques da Silva, María Florencia Eberhardt, Nahuel A. Riviere, David Gally, Shannon D. Manning, Angel CataldiA) Evidence and graphic representation primers for combinatorial PCR to evaluate inversion. B) In silico prediction of amplicons using assembled genomes. C) Agarose gel of amplified fragments. All primers pairs were tested on 2 isolates from 7.1Anguil and one from RafaelaIIA.
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whole genome sequencingshows key differencessevere systemic diseaseprotease espp geneprophage replication proteinsp γ alleleshemolytic uremic syndromegenes exclusively founddna binding proteinsdiv >< pmolecular bases relatedmain causative agentescherichia coli o157 strain variationcomparative genomic analysish7 strains circulatingh7 isolates recoveredshiga toxin stx2aclade 8 strainsshiga toxingenomic analysisstx2a main reservoirhighly relatedclade 8h7 isolatedstudied strainsfour strainswork alignstransposon interruptionstx2a productionpo157 plasmidobserved variabilitymostly encodedintergenic regionhus ).humid pampashighly diversegenetic basisdistinctive serotypedifferent levelscontained 13clade 6better understand000 children
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