posted on 2024-01-02, 13:35authored bySinda Lepetre-Mouelhi, Fréderic Gobeaux, Alexandre Da Silva, Lucas Prades, Jiao Feng, Frank Wien, Patrick Couvreur, Fabienne Testard
Recently,
we proposed in a paper a novel nanomedicine approach
based on a versatile bioconjugation linkage (amide, diglycolate, or
acylal) between the Leu-enkephalin (LENK), an endogenous neuropeptide,
and the squalene (SQ), a natural and biocompatible lipid. The nanoformulation
of the resulting bioconjugates allowed the specific delivery of LENK
into inflamed tissues for efficient pain control after their intravenous
administration. However, the variability of the analgesic profiles
exhibited by the three types of LENK-SQ nanoparticles (NPs) remains
not well understood. This study investigates the influence of the
LENK-SQ NP composition on their supramolecular organization and their
interaction with blood proteins. To achieve this objective, a physicochemical
study was carried out using complementary techniques such as dynamic
light scattering (DLS), small/wide-angle X-ray and neutron scattering
(SAXS-WAXS and SANS), and circular dichroism (CD). This study brought
together some important information, allowing us to shed light on
the crucial link between nanoparticles’ structure, stability,
and analgesic activity.