posted on 2024-02-07, 21:14authored byDonghui Wu, Qilin Zhao, Bing Zhang, Xianqing Tang, Yushu Li, Jian Sun, Xiurong Yang
Nanozymes
with multiple functionalities endow biochemical sensing
with more sensitive and efficient analytical performance by widening
the sensing modes. Meanwhile, the target-oriented design of multifunctional
nanozymes for certain biosensing remains challenging. Herein, a constructive
strategy of doping iron into polymer dots (PDs) to achieve nanozymes
with excellent oxidase-mimicking and peroxidase-mimicking activity
is proposed. Compared with the Fe-free PDs prepared under the same
mild condition, the Fe-doped PDs (Fe-PDs) exhibit greatly boosted
fluorescence at 500 nm. While applying 3,3′,5,5′-tetramethylbenzidine
(TMB) as a chromogenic substrate, the fluorescence of the Fe-PDs can
be further quenched by oxTMB due to the inner filter effect (IFE).
Inspired by this, a simple but efficient colorimetric and fluorometric
dual-mode sensing platform is developed for monitoring the reducing
substances ascorbic acid (AA), α-glucosidase (α-Glu),
and its inhibitors (AGIs). We believe that such multifunctional enzyme-mimic
materials will provoke the exploration of multimode sensing strategy
with strong practicality to serve as a versatile tool in biochemical
sensing.