salini-intestinaltranscriptome-2020.pdf (3.58 MB)
Intestinal Transcriptome Analysis Highlights Key Differentially Expressed Genes Involved in Nutrient Metabolism and Digestion in Yellowtail Kingfish (Seriola lalandi) Fed Terrestrial Animal and Plant Proteins
journal contribution
posted on 2020-01-01, 00:00 authored by Chinh Thi My Dam, Tomer Ventura, Mark Booth, Igor Pirozzi, Michael SaliniMichael Salini, Richard Smullen, Abigail ElizurThis study investigated the effects of dietary terrestrial animal and plant proteins on the intestinal transcriptomes of yellowtail kingfish (YTK), Seriola lalandi, an ecologically and economically important marine species in Australia. Five diets containing fish meal (FM), poultry by-product meal (PBM), blood meal (BLM), faba bean meal (FBM) and corn gluten meal (CGM) were formulated and fed over a period of 4 weeks. The Illumina RNA-sequencing (RNA-Seq) results identified a suite of differentially expressed genes involved in nutrient metabolism and protein digestion pathways, reinforced by quantitative polymerase chain reaction (qPCR) results. These findings provide molecular support to the notion that PBM and FBM are useful raw materials in commercial diets for YTK. Using the same evidence, we have demonstrated that BLM and CGM may be less useful and their incorporation into commercial aquafeeds for this species should be done cautiously. The differentially expressed genes showed a subtle difference and high correlation with apparent nutrient digestibility of raw materials. Further, our results indicate that transcriptome profiling provides a useful tool to evaluate alternative protein sources for use in aquaculture feeds.
History
Journal
GenesVolume
11Issue
6Article number
621Pagination
1 - 17Publisher
MDPI AGLocation
Basel, SWitzerlandPublisher DOI
Link to full text
eISSN
2073-4425Language
engPublication classification
C1.1 Refereed article in a scholarly journalUsage metrics
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Keywords
Science & TechnologyLife Sciences & BiomedicineGenetics & Heredityyellowtail kingfishRNA-sequencingtranscriptomesalternative protein sourcesdistal intestinedigestionBY-PRODUCT MEALSALMON SALMO-SALAREUROPEAN SEA-BASSFISH-MEALSOYBEAN-MEALRAINBOW-TROUTATLANTIC SALMONTAURINE SUPPLEMENTATIONTOTAL REPLACEMENTFEED-UTILIZATIONGenetics
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