posted on 2013-09-12, 00:00authored byAyumi Sumino, Takehisa Dewa, Tomoyasu Noji, Yuki Nakano, Natsuko Watanabe, Richard Hildner, Nils Bösch, Jürgen Köhler, Mamoru Nango
In
the photosynthetic membrane of purple bacteria networks of light-harvesting
2 (LH2) complexes capture the sunlight and transfer the excitation
energy. In order to investigate the mutual relationship between the
supramolecular organization of the pigment–protein complexes
and their biological function, the LH2 complexes were reconstituted
into three types of phospholipid membranes, consisting of l-α-phosphatidylglycerol (PG), l-α-phosphatidylcholine
(PC), and l-α-phosphatidylethanolamine (PE)/PG/cardiolipin
(CL). Atomic force microscopy (AFM) revealed that the type of phospholipids
had a crucial influence on the clustering tendency of the LH2 complexes
increased from PG over PC to PE/PG/CL, where the LH2 complexes formed
large, densely packed clusters. Time-resolved spectroscopy uncovered
a strong quenching of the LH2 fluorescence that is ascribed to singlet–singlet
and singlet–triplet annihilation by an efficient energy transfer
between the LH2 complexes in the artificial membrane systems. Quantitative
analysis reveals that the intercomplex energy transfer efficiency
varies strongly as a function of the morphology of the nanostructure,
namely in the order PE/PG/CL > PC > PG, which is in line with
the
clustering tendency of LH2 observed by AFM. These results suggest
a strong influence of the phospholipids on the self-assembly of LH2
complexes into networks and concomitantly on the intercomplex energy
transfer efficiency.