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Independent infection time series using GFP switch-on cross-kingdom RNAi reporter plant seedlings infected with B. cinerea WT, bcrdr1 ko mutant #2 or water-treated.

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posted on 2023-12-20, 18:44 authored by An-Po Cheng, Bernhard Lederer, Lorenz Oberkofler, Lihong Huang, Nathan R. Johnson, Fabian Platten, Florian Dunker, Constance Tisserant, Arne Weiberg

A) Fluorescence microscopy images at different time points of infection. A 5 μl drop of a 2 x 105/ml conidiospore suspension was placed at the center of the leaf before placing a glass covering slip on the top that dispersed the spore suspension and led to GFP activation over the entire leaf. The scale bars represent 1 mm. B) Normalized GFP signal quantification of whole seedling leaves over the time series of 6–42 hpi.

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