pbio.3002544.s005.pdf (3.82 MB)
Increasing concentrations of IsoT in minigenome assay partially affects polymerase activity.
journal contribution
posted on 2024-02-29, 18:53 authored by Carlos A. Rodríguez-Salazar, Sarah van Tol, Olivier Mailhot, Maria Gonzalez-Orozco, Gabriel T. Galdino, Abbey N. Warren, Natalia Teruel, Padmanava Behera, Kazi Sabrina Afreen, Lihong Zhang, Terry L. Juelich, Jennifer K. Smith, María Inés Zylber, Alexander N. Freiberg, Rafael J. Najmanovich, Maria I. Giraldo, Ricardo Rajsbaum(A–C) HEK293T cells were transfected with minigenome components and (A) including 12.5, 25, and 50 ng of IsoT WT. (B, C) Including 50, 100, and 200 ng of IsoT WT or catalytic impaired mutant IsoT 335A, 50 h later cells were lysed for luciferase assay and western blot analysis. The data underlying the graphs shown in the figure can be found in S1 Data.
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