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In silico analysis and characterization of fresh water fish ATPases and homology modelling
journal contribution
posted on 2017-11-24, 12:01 authored by Rumpi Ghosh, AD Upadhayay, AK RoyATPases is known to be a crucial in many biological activities of
organisms. In this study, physicochemical properties and modeling of
ATPases protein of fish was analysed using In silico approach. ATPases a protein selected from fish species, including Gold fish (Carassius auratus auratus), Zebra fish (Hypancistrus zebra), White fishes (Coregonus autumnalis), Grass carp (Ctenopharyngodon idella) and Anabas testudineus
(Koi) were used in this study. Physicochemical characteristics showed
with molecular weight (25045.58-25148.57Da), theoretical isoelectric
point (9.30-9.97), extinction coefficient(26470-34950), aliphatic
index(147.31-150.35), instability index(32.84-42.67), total number of
negatively charged residues and positively charged residues (5/7-6/8),
and grand average of hydropathicity (1.014-1.151) were computed. All
proteins were classified as transmembrane proteins. In secondary
structure prediction, all proteins were composed of random coils as
predominant, followed by extended strands, alpha helix and beta turn.
Three dimensional structure of protein were predicted and verified as
good structures. All model structures were evaluated being accepted and
reliable based on structural evaluation and stereo chemical analysis.
