ppat.1009891.s002.docx (471.97 kB)
Immunoblotting for RORγt in thymic cells from wild-type and RORγt-/- mice.
journal contribution
posted on 2021-08-31, 17:32 authored by Bhawna Yadav, Charles A. Specht, Chrono K. Lee, Maria Pokrovskii, Jun R. Huh, Dan R. Littman, Stuart M. LevitzThymic cells were isolated from thymuses harvested from wild-type (WT) and RORγt-/- (KO) mice. Cytoplasmic and nuclear extracts were prepared by cellular fractionation, resolved by SDS-PAGE, and analyzed by immunoblotting with an antibody directed against RORγt. Migration of the molecular size markers (in kDa) is shown on the right. The expected size of the RORγt band is 58 kDa. The immunoblot is representative of 2 separate experiments.
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sup >-/-mcherry reporter mousemaster transcription factor>- challenged wildutilized murine modelslung eosinophils elicitedcomparing lung eosinophilsdiv >< pacute pulmonary aspergillosiseosinophil rorγt expressionacute modelspulmonary aspergillosiseosinophil ilwestern blottingpositively correlatedlive conidiaimmunocompetent personsformer predominatedflow cytometrycytoplasmic localizationconfocal microscopyatopic individualsadverse consequences
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