Identifying the Role of Flagella in the Transport of Motile and Nonmotile Salmonella enterica Serovars

The influence of bacterial flagella on cell transport has been examined using three Salmonella enterica serovars with different motility phenotypes. Both a packed bed (PB) column and radial stagnation point flow system (RSPF) were employed to investigate bacterial deposition kinetics onto quartz over a range of conditions. The aim of this study was to better understand the role of motility on the deposition behavior of Salmonella, an important food- and waterborne pathogen. In both the PB column and RSPF systems, flagellated strains showed higher deposition, indicating the nonmotile cells can be transported further than the motile. At late-exponential growth phase, the deposition of the functional flagellated motile strain was enhanced, much more so than the dysfunctionally flagellated nonmotile and nonflagellated strains, highlighting the role of flagellar motion in cellular deposition and retention through available pore spaces and irregularities on quartz surfaces.