Identification of high-value bioactive constituents in Northern European willow varieties: S.X. Dasyclados, Endeavour, Cheviot, Tora, Resolution, S. Purpurea, Terranova, endurance

Willow trees have maintained their place in medicine for many years as they are herbal source of various cures.  Willow varieties are considered to contain a wide range of anti-inflammatory and anti-bacterial species such as  salicylates and flavonoids. The current work is centred on the presence of high bioactive pharmaceutical constituents other than salicin such as flavan-3-ol catechin, salicortin, and other complex compounds, which  contribute to the total medical value of willow extracts. To evaluate the distribution of these bioactives, bark, and  wood fractions of 8 different willow varieties (S.X. Dasyclados, Endeavour, Cheviot, Tora, Resolution, S. Purpurea,  Terranova, Endurance) were extracted using dispersive solid phase extraction and then analysed using LC-MS  comprising a quadrupole time-of-flight spectrometer. Indeed, various high value constituents such as salicortin, catechin, triandrin, acacetin-5-O-xyloside, picein, apigenin-7-O-glucoside, vitexin-2-rhamnoside, luteolin7-glucoside, catechin gallate, and kaempferol as well as giberellic and 5-methoxysalicylic acid were detected in  bark and wood fractions of the willow varieties 80:20 ethanol/water extracts.