posted on 2022-01-20, 12:03authored byYaofeng Chen, Sujuan Zhao, Ran Ding, Hui Li, Cai-Xia Yang, Zhi-Qiang Du
Long
noncoding RNAs are vital to a variety of biological and physiological
processes through multiple modes of functional interaction with DNA,
RNA, and proteins. In chickens, numerous lncRNAs were discovered to
be important to growth or disease progression. However, the detailed
molecular function and role of lncRNAs remain less explored. Here,
we performed lncRNA sequencing on abdominal adipose tissues from broiler
lines divergently selected for abdominal fat content, and significantly
differentially expressed lncRNAs were found, including lncPRDM16,
a divergently transcribed and conserved lncRNA near PRDM16. Full lengths
of two transcripts of lncPRDM16 were obtained, and their genomic structures
were compared. Expression dynamics of lncPRDM16 in different tissues
and during preadipocyte proliferation and differentiation were profiled.
Moreover, a 250-nucleotide sequence at 5′-end was found to
be inevitable to the function of lncPRDM16 in inhibiting preadipocyte
proliferation and regulating the promoter activities of both lncPRDM16
and PRDM16. Taken together, we identified the 5′-end functional
elements of lncPRDM16 and their potential importance in inhibiting
preadipocyte proliferation. Our findings provide the foundation for
further exploration of lncPRDM16 function and potential improvement
of chicken muscle quality.