IPTG titration for the P_lac-dnaA strain.

(A) DnaA immunoblot and respective quantification from wild-type and P_lac-dnaA cells sampled at 4 hours after being shifted to M2G medium containing the indicated IPTG concentrations. Before shift, cells were cultivated until mid-exponential phase in M2G 50 μM IPTG. (B) Length measurements of 800 cells sampled as in (A). Lines and error bars indicate means ± SD. The extended horizontal line emphasizes the average length of wild-type cells in the absence of IPTG. (C) Micrographs and flow cytometry DNA content histograms (of 30 000 cells each) from cells sampled as in (A). Scale bars: 5 μm.

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