IF detection of eGFP in embryonic ovaries and mature testis.

Weitzel, Alexander J.; Grunwald, Hannah A.; Weber, Ceri; Levina, Rimma; Gantz, Valentino M.; Hedrick, Stephen M.; Bier, Ethan; Cooper, Kimberly L.

doi:10.1371/journal.pbio.3001478.s004

pbio.3001478.s004.pdf (739.04 kB)

IF detection of eGFP in embryonic ovaries and mature testis.

journal contribution

posted on 2021-12-23, 18:48 authored by Alexander J. Weitzel, Hannah A. Grunwald, Ceri Weber, Rimma Levina, Valentino M. Gantz, Stephen M. Hedrick, Ethan Bier, Kimberly L. Cooper

(A-C) Wild-type embryonic ovary. All other panels in each row are Spo11^{Cas9-P2A-eGFP/+} littermates (e.g., A’–A”‘). (D) Wild-type and (D’) Spo11^{Cas9-P2A-eGFP/+} adult testis. Scale bar is 50 μm for all panels. Confocal fluorescence imaging settings are identical for all samples; eGFP expression is substantially higher in testes compared to any embryonic ovary time point. IF, immunofluorescence.

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