posted on 2022-12-20, 18:36authored byChenzhipeng Nie, Ben Wang, Mingcong Fan, Yu Wang, Yujie Sun, Haifeng Qian, Yan Li, Li Wang
The tea of roasted Highland barley is a cereal-based
drink rich
in polyphenols. A model of skeletal muscle senescence and fibrosis
was constructed using d-galactose-induced C2C12 myotubes,
and Highland barley tea Polyphenols (HBP) were extracted for the intervention.
We found that HBP effectively alleviated oxidative stress, inflammation,
and fibrosis induced by d-galactose-induced skeletal muscle
senescence. Also, HBP treatment significantly down-regulated pro-fibrotic
genes, inflammation, and oxidative stress levels in a contusion model
of senescent mice. Reduced levels of SIRT3 protein was found to be
an essential factor in skeletal muscle senescence and fibrosis in
both cellular and animal models, while HBP treatment significantly
increased SIRT3 protein levels and viability in skeletal muscle. The
ability of HBP to mitigate skeletal muscle fibrosis and oxidative
stress was significantly reduced after SIRT3 silencing.
Together, these results suggest that HBP intervention can significantly
alleviate aging-induced oxidative stress, inflammation, and skeletal
muscle fibrosis, with the activation of SIRT3 as
the underlying mechanism of action.