posted on 2023-11-21, 11:13authored byYumeng Wang, Gan Zhang, Xiaoyue Xiao, Xinhui Shu, Dan Fei, Yelan Guang, Yaomin Zhou, Weihua Lai
The label with a large Stokes shift and strong fluorescence
properties
could improve the sensitivity of the lateral flow immunoassay (LFIA).
Herein, two aggregation-induced emission (AIE) luminogens with spectral
overlap were encapsulated in polymers by using the microemulsion method
as a label, and the construction of a fluorescence resonance energy
transfer mode was further verified via theoretical calculation and
spectral analysis. Satisfactorily, the doped AIE polymer microspheres
(DAIEPMs) exhibited a large Stokes shift of 285 nm and a 10.8-fold
fluorescence enhancement compared to those of the AIEPMs loaded with
acceptors. Benefiting from the excellent optical performance, DAIEPMs
were applied to the LFIA for sensitive detection of chlorothalonil,
which is an organochlorine pesticide. The limit of detection of the
proposed DAIEPMs-LFIA was 1.2 pg/mL, which was 4.8-fold and 11.6-fold
lower than those of quantum dot bead LFIA and gold nanoparticle LFIA,
respectively. This work provides a new strategy to improve the optical
properties of fluorescent materials and construct a sensitive and
reliable detection platform.