pbio.3001478.s003.pdf (2.94 MB)
Hematoxylin and eosin staining of testes.
journal contribution
posted on 2021-12-23, 18:48 authored by Alexander J. Weitzel, Hannah A. Grunwald, Ceri Weber, Rimma Levina, Valentino M. Gantz, Stephen M. Hedrick, Ethan Bier, Kimberly L. Cooper(A)Spo11Cas9-P2A-eGFP/+ and (B) Spo11Cas9-P2A-eGFP/Cas9-P2A-eGFP seminiferous tubules; scale bar is 50 μm. Cells in seminiferous tubules in (B) likely do not complete meiosis, evidenced by absence of sperm. (C) Tiled image of Spo11Cas9-P2A-eGFP/Cas9-P2A-eGFP testis; scale bar is 200 μm. All seminiferous tubules are deformed and devoid of sperm.
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wild rodent populationsstrand dna breaksequence targeted crisprdisease transmission causedfemale germlines suggestsmendelian ” inheritancefemale mouse germlineefficient dsb formationdiv >< ptiming cas9 expressionfemale germlinemeiotic expressiondeletion ”“ super“ insertionspo11 previously showedobservations suggestedmay improvelimit lossinterchromosomal homologyinstead repairedhomologous chromosomesgene conversionfavor conditionsend joiningdirected repaircas9 maycas9 knockcas9 double
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