H₂S production in deletion mutants of V. cholerae cbs, cse, and 3mst candidates.

Overnight cultures of single deletion for homologs of cbs, cse, and 3mst were 1:100 sub-cultured into fresh Luria–Bertani broth containing 500 μM L-cysteine hydrochloride, followed by stationary incubation at 37°C for 18 hrs. H₂S production was measured by the darkening of Pb(Ac)₂ paper stripe during cultivation. The average H₂S level of the wild-type was set to be 100% for subsequent normalization. Three replicates were sampled for each strain. Significance was determined by t-test; p-value: ***, <0.001.

(PDF)