Growth plate analysis of S. aureus mutant strains with transposon insertions in genes identified as essential for growth at pH 4.5.

(A-H) Bacterial growth on TSA pH 7.3 plates. Overnight cultures of the indicated WT and mutant strains were serially diluted and spotted on TSA pH 7.3 plates. Images were taken following 24 h incubation at 37°C. Each image is a representative of three experiments.

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