ppat.1012050.s005.pdf (410.37 kB)
Growth curves and corresponding area under the curve analysis for the strains utilized for the metabolic flux assay shown in Fig 4E.
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posted on 2024-02-29, 18:55 authored by Sydney Stanley, Xin Wang, Qingyun Liu, Young Yon Kwon, Abigail M. Frey, Nathan D. Hicks, Andrew J. Vickers, Sheng Hui, Sarah M. FortuneAll cultures started at OD600 0.005 at day 0. Triplicate replicates shown, error bars represent the standard deviation. H37Rv carries the -18 G>T lldD2 mutation. KanR refers to kanamycin resistance. P-values indicate the results of unpaired t-tests. Representative of two independent experiments.
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providing experimental evidencediv >< pcentral carbon metabolism1 virulence systemmycobacterium tuberculosis discernible growth defectdependent antibiotic susceptibilitymutations functionally increasehuman host environmentglobal clinical isolateslactate dehydrogenase revealsassessing mtb genes13 clinical isolatesvivo mtb growthlactate dehydrogenaseintracellular environmenthost pressuregenes involveddependent lvariation acrosstranscriptionally sensitivesugar phosphatesstrongest targetsstepwise natureselective advantagesalient featurerobust incorporationprovides contextpressures facingpoorly characterizedpleiotropic effectsmycobacterial cellmultifunctional rolemtb genomelldd2 lactate accumulationfunctional reporterdetoxification benefitdeleterious buildupdefining mutationsdata illustratebacterial determinantsbacterial adaptionancestral lineage
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