posted on 2021-08-05, 19:36authored byShovon Bhattacharjee, Rakesh Joshi, Muhammad Yasir, Anurag Adhikari, Abrar Ahmad Chughtai, David Heslop, Rowena Bull, Mark Willcox, Chandini Raina Macintyre
Protection
against pathogens using personal protective equipment is essential
yet challenging
in healthcare settings. Concerns over emerging biothreats and outbreaks
of infectious diseases underscore the need for antimicrobial and biocompatible
protective clothing to protect patients and staff. Herein, we report
the antimicrobial efficacy and cytotoxicity of cotton/silk fabrics
containing embedded reduced graphene oxide (RGO) and Ag/Cu nanoparticles
(NPs), prepared using a 3-glycidyloxypropyl trimethoxy silane coupling
agent followed by chemical reduction and vacuum heat treatment. Embedding
NPs on top of the RGO layer substantially increased the antimicrobial
activity. All RGO–Ag NPs or RGO–Cu NPs embedded in cotton
or silk fabrics reduced the viability of approximately 99%
of the Gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa. RGO–Ag
NPs embedded into cotton or silk fabrics reduced the viability of
the Gram-positive bacterium Staphylococcus aureus by 78–99%, which was higher than the growth inhibition by
RGO–Cu NPs samples against S. aureus. Both silk and cotton containing RGO–Cu NPs produced a greater
reduction in the viability of the yeast Candida albicans compared to RGO–Ag NPs fabrics. All RGO–Ag NPs or
RGO–Cu NPs embedded in cotton or silk fabrics showed good washing
durability by sustaining good bactericidal activity, even on washing
up to 10 times. Moreover, none of the RGO–Ag or RGO–Cu
fabrics reduced mammalian cells’ (HEK293) viability by >30%,
suggesting low cytotoxicity and good biocompatibility. These findings
show that RGO–NPs embedded in cotton or silk fabrics have great
potential for use in protective clothing and medical textiles.