Genotyping strategy for Spo11^{Cas9-P2A-eGFP}.

(A) Schematic of primer binding location for PCR genotyping. (B) Gel depicting PCR of Spo11 locus revealing the genotype of Spo11^+/+, Spo11^{Cas9-P2A-eGFP/+}, and Spo11^{Cas9-P2A-eGFP/Cas9-P2A-eGFP}. The raw gel image in (B) can be found at the associated Zenodo data repository (https://doi.org/10.5281/zenodo.5510697) in the file labeled “S1 Raw Images.”

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