Gene replacement of BIP1.

A. M. oryzae BIP1 locus regions used to construct the gene replacement vector. 1.2 kb and 1.36 kb genomic regions (respectively Left Border and Right Border grey boxes) flanking the BIP1 ORF were amplified using P1.2 genomic DNA and primers shown as arrows (S5 Table). The four exons of BIP1 are shown as black boxes separated by introns. B. Structure of the BIP1 locus in the Δbip1 mutants. Hatched boxes correspond to the hygromycin resistance cassette. Grey boxes represent the Left and Right Border sequences flanking the BIP1 ORF used to construct the gene replacement vector. C. Analysis of the transformants by Southern blot. Genomic DNA was digested with HindIII and probed with the 1.36 kb RB fragment (top) and 0.85 kb hph cassette (bottom). Lanes 1, 2, and 3, Δbip1 transformants; lane 4, Wild type (P1.2).

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