ppat.1011585.s005.pdf (62.61 kB)
Gating used to separate iRBC from NK cells and identify free PV.
journal contribution
posted on 2023-11-08, 18:49 authored by Padmapriya Sekar, Sumati Rajagopalan, Estela Shabani, Usheer Kanjee, Marc A. Schureck, Gunjan Arora, Mary E. Peterson, Boubacar Traore, Peter D. Crompton, Manoj T. Duraisingh, Sanjay A. Desai, Eric O. LongEXP2-GFP+ iRBC were stained with eFluor 670 and membrane dye PKH26. NK cells were stained with PKH67. iRBC were gated away from NK cells first by forward and side scatter, and then gated for eFluor670+ PKH67-negative cells, followed by gating the PKH26-negative eFluor670+ population consisting of free PV.
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within parasitophorous vacuolessup >+releases parasitophorous vacuolesquantitative flow cytometrymechanism may mitigatelytic effector moleculesinducible erythrocyte precursorsfree vesicles similarelectron microscopy imagesapplied gene ablationpeople chronically exposeddiv >< pcells lyse virusmonocytes may combinedependent cellular cytotoxicityplasmodium falciparum nk cells lysenk cells bindadaptive nk cellsrbc plasma membranep falciparum nk cellstransformed cellstarget cellsinfected cellsexposed individualspv membraneselective nknk celltwo orderssubsequent phagocytosisspecific recognitionreleased intactreduced parasitemiaprimary monocytespolarized deliverynatural killermediated adccmagnitude higherlive imagingintegrin αmβ2high frequencyefficient killingcentral rolecell sortingblocking experimentsadcc ).
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