ppat.1011585.s002.pdf (198.25 kB)
Gating to identify iRBC and a control for damage to iRBC in the absence of NK cells.
journal contribution
posted on 2023-11-08, 18:49 authored by Padmapriya Sekar, Sumati Rajagopalan, Estela Shabani, Usheer Kanjee, Marc A. Schureck, Gunjan Arora, Mary E. Peterson, Boubacar Traore, Peter D. Crompton, Manoj T. Duraisingh, Sanjay A. Desai, Eric O. Long(A) Gating of eFluor 670+ glycophorin A (CD235a)+ iRBC and LFA-1+ NK cells to determine damage to iRBC with Phalloidin-AF405 and to the parasite with PI. (B) Same analysis as in (A) of iRBC incubated for 3 hours with anti-RBC IgG in the absence of NK cells. (C) Representative experiment performed as in Fig 2C performed in the absence of NK cells.
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