Gating strategy for the detection of different immune cell subsets from malignant ascites of ovarian cancer patients.

Yoshida-Court, Kyoko; Karpinets, Tatiana V.; Mitra, Aparna; Solley, Travis N.; Dorta-Estremera, Stephanie; Sims, Travis T.; Medrano, Andrea Y. Delgado; Alam, Molly B. El; Ahmed-Kaddar, Mustapha; Lynn, Erica J.; Sastry, K. Jagannadha; Zhang, Jianhua; Futreal, Andrew; Nick, Alpa; Lu, Karen; Colbert, Lauren E.; Klopp, Ann H.

doi:10.1371/journal.pone.0279590.s001

pone.0279590.s001.pdf (124.96 kB)

Gating strategy for the detection of different immune cell subsets from malignant ascites of ovarian cancer patients.

journal contribution

posted on 2023-01-06, 18:40 authored by Kyoko Yoshida-Court, Tatiana V. Karpinets, Aparna Mitra, Travis N. Solley, Stephanie Dorta-Estremera, Travis T. Sims, Andrea Y. Delgado Medrano, Molly B. El Alam, Mustapha Ahmed-Kaddar, Erica J. Lynn, K. Jagannadha Sastry, Jianhua Zhang, Andrew Futreal, Alpa Nick, Karen Lu, Lauren E. Colbert, Ann H. Klopp

Cells collected from ascites were stained with a 9-color flow cytometry panel and analyzed in an X-20 Fortessa flow cytometer. (A) The gating strategy is depicted starting with the detection of lymphocytes, followed by live cells and CD3+ T cells that are separated according to CD4 and CD8 expression. (B) Fluorescence-minus-one (FMO) staining is shown for the different markers analyzed on CD4+ and CD8+ T cells.

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