ppat.1011585.s001.pdf (107.64 kB)
Flow cytometry profiles of EJ cells and RBC.
journal contribution
posted on 2023-11-08, 18:49 authored by Padmapriya Sekar, Sumati Rajagopalan, Estela Shabani, Usheer Kanjee, Marc A. Schureck, Gunjan Arora, Mary E. Peterson, Boubacar Traore, Peter D. Crompton, Manoj T. Duraisingh, Sanjay A. Desai, Eric O. Long(A) Flow cytometry profiles of cell surface staining using Rabbit anti-RBC polyclonal antibody in uninfected and 3D7-infected RBC. (B) Flow cytometry profiles of cell surface staining of Cas9 control, ICAM4 KO and CD58 KO EJ cells for ICAM4 and CD58, showing the loss of ICAM4 and CD58 in the respective knockout cells.
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within parasitophorous vacuolessup >+releases parasitophorous vacuolesquantitative flow cytometrymechanism may mitigatelytic effector moleculesinducible erythrocyte precursorsfree vesicles similarelectron microscopy imagesapplied gene ablationpeople chronically exposeddiv >< pcells lyse virusmonocytes may combinedependent cellular cytotoxicityplasmodium falciparum nk cells lysenk cells bindadaptive nk cellsrbc plasma membranep falciparum nk cellstransformed cellstarget cellsinfected cellsexposed individualspv membraneselective nknk celltwo orderssubsequent phagocytosisspecific recognitionreleased intactreduced parasitemiaprimary monocytespolarized deliverynatural killermediated adccmagnitude higherlive imagingintegrin αmβ2high frequencyefficient killingcentral rolecell sortingblocking experimentsadcc ).
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