Flow cytometry analysis of early and late apoptotic cells.

Serra, Riccardo; Zhao, Tianna; Huq, Sakibul; Gorelick, Noah Leviton; Casaos, Joshua; Cecia, Arba; Mangraviti, Antonella; Eberhart, Charles; Bai, Renyuan; Olivi, Alessandro; Brem, Henry; Jackson, Eric M.; Tyler, Betty

doi:10.1371/journal.pone.0251957.s003

pone.0251957.s003.pdf (1.76 MB)

Flow cytometry analysis of early and late apoptotic cells.

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posted on 2021-11-03, 17:24 authored by Riccardo Serra, Tianna Zhao, Sakibul Huq, Noah Leviton Gorelick, Joshua Casaos, Arba Cecia, Antonella Mangraviti, Charles Eberhart, Renyuan Bai, Alessandro Olivi, Henry Brem, Eric M. Jackson, Betty Tyler

AnnV/PI staining showed a significant increase in AnnV⁺-PI^- and AnnV⁺-PI⁺ cells after 12-24h and 24-48h, respectively. DSF-Cu⁺⁺ was used at a concentration of 150nM in all cell lines. SSC/FSC gating is shown for each cell line, as well as AnnV/PI gating in control and DSF-Cu⁺⁺-treated samples.

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Flow cytometry analysis of early and late apoptotic cells.

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