ab1c00732_si_001.pdf (1.84 MB)
Fast Identification and Quantification of Uropathogenic E. coli through Cluster Analysis
journal contribution
posted on 2021-12-13, 10:29 authored by Jean-Baptiste Vendeville, Mathew John Kyriakides, Yuiko Takebayashi, Sylvain Rama, James Preece, Jenny Samphire, Javier Ramos-Soriano, Abraham Mauleon Amieva, Maisie Emma Holbrow-Wilshaw, Henry Ralph Gordon Newman, Sio Lou Kou, Sandra Medina-Villar, Neciah Dorh, Josephine Ndoa Dorh, James Spencer, M. Carmen GalanRapid
diagnostic
tools to detect, identify, and enumerate bacteria
are key to maintaining effective antibiotic stewardship and avoiding
the unnecessary prescription of broad-spectrum agents. In this study,
a 15 min agglutination assay is developed that relies on the use of
mannose-functionalized polymeric microspheres in combination with
cluster analysis. This allows for the identification and enumeration
of laboratory (BW25113), clinical isolate (NCTC 12241), and uropathogenic Escherichia coli strains (NCTC 9001, NCTC 13958,
J96, and CFT073) at clinically relevant concentrations in tryptic
soy broth (103–108 CFU/mL) and in urine
(105–108 CFU/mL). This fast, simple,
and efficient assay offers a step forward toward efficient point-of-care
diagnostics for common urinary tract infections.
