pone.0299294.s002.pdf (127.86 kB)
Extended materials and methods for all extracellular flux analysis assays: Modified mitochondrial stress test, iNOS inhibitor kinetic assay, and glycolytic burst assay.
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posted on 2024-03-07, 18:39 authored by Bay Vagher, Eyal AmielExtended materials and methods for all extracellular flux analysis assays: Modified mitochondrial stress test, iNOS inhibitor kinetic assay, and glycolytic burst assay.
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offer novel insightlarge scale changeskey events includinggene expression includingbiosynthetic metabolic intermediatesacute metabolic reprogrammingmetabolism favoring glycolysismediated metabolic effectsinos reaction kineticallydc activation processreal time detectionmediated effectsreal timetime assaydc metabolismcellular metabolismusing realsingle realtemporal relationshipspecific timepointsrapid inductionnitric oxidemitochondrial respirationinextricably linkedfully describedfindings provideexperimentally establishdiscuss parametersdependent mannerbetter understandingactivating stimulus
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