Expression of M-Sec in CD3⁺CD4⁺CADM1⁺ cells.

(A) The CD3⁺CD4⁺CADM1⁺ cells in the live cell gate were sorted from PBMCs of HTLV-1 carriers. The profile of an HTLV-1 carrier is shown as an example. (B) The CD3⁺CD4⁺CADM1⁺ cells sorted from PBMCs of an HTLV-1 carrier were cultured for 3 days, and analyzed for M-Sec (green) and CD3 (red). The nuclei were also stained with DAPI (blue). Monocytes were added as a positive control for M-Sec. The antibodies used for staining were as follows: anti-M-Sec (F-6; Santa Cruz Biotechnology), and anti-CD3 (CD3-12; Abcam). Scale bar: 10 μm. (C) The cells were analyzed as in (B), and the percentages of CD3⁺CD4⁺CADM1⁺ cells or monocytes expressing M-Sec at a detectable level are shown (15 cells for each). The typical signal of monocytes was defined as “bright”.

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