ppat.1009891.s001.docx (1.03 MB)
Eosinophil gating strategy.
journal contribution
posted on 2021-08-31, 17:32 authored by Bhawna Yadav, Charles A. Specht, Chrono K. Lee, Maria Pokrovskii, Jun R. Huh, Dan R. Littman, Stuart M. LevitzTotal lung cells were prepared from mice with allergic aspergillosis using the MACS lung dissociation kit (Miltenyi Biotec). Following staining, cells were analyzed by flow cytometry. Eosinophils were defined based on the sequential gating strategy shown in the figure. FSC, forward scatter. SSC, side scatter.
(DOCX)
History
Usage metrics
Categories
Keywords
sup >-/-mcherry reporter mousemaster transcription factor>- challenged wildutilized murine modelslung eosinophils elicitedcomparing lung eosinophilsdiv >< pacute pulmonary aspergillosiseosinophil rorγt expressionacute modelspulmonary aspergillosiseosinophil ilwestern blottingpositively correlatedlive conidiaimmunocompetent personsformer predominatedflow cytometrycytoplasmic localizationconfocal microscopyatopic individualsadverse consequences
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC