ppat.1010138.s002.docx (393.98 kB)
Endogenous C-terminal tagging of TgGRA14 in a type II strain.
journal contribution
posted on 2021-12-13, 18:36 authored by Yolanda Rivera-Cuevas, Joshua Mayoral, Manlio Di Cristina, Anna-Lisa E. Lawrence, Einar B. Olafsson, Romir K. Patel, Dishari Thornhill, Benjamin S. Waldman, Akira Ono, Jonathan Z. Sexton, Sebastian Lourido, Louis M. Weiss, Vern B. CarruthersA. Schematic for the tagging strategy with primer binding sites. B. PCR to validate the tagging of TgGRA14 with HA in the ME49Δku80 background. C. Representative images showing the co-localization of HA with TgGRA14 in the tagged strain. Scale bar is 5 μm.
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master manipulator capablelate domain motifsfusogenic parasitophorous vacuolecontains motifs homologousparasite proteins includinghost cytosolic proteinsdense granule proteinsdeficient parasites revealedhost escrt componentsparasite gains resourcestoxoplasma gondii dependent vlp buddinghost escrt machineryparasite uptakehost cytosolhost cellwt parasitesvesicular buddinggondii dense granulesmediate escrtresiding withinreplicative nicherelease assaypvm ).molecular underpinningsmarked reductionlimiting membranelike particlekey pathwayintracellular subsistenceeffectively siphoningcontaining portionalso showaccesses contents1 virus1 gag
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