pntd.0009071.s002.docx (323.17 kB)
Endo H cleavage of G. morsitans salivary glycoproteins.
journal contribution
posted on 2021-02-02, 18:43 authored by Radoslaw P. Kozak, Karina Mondragon-Shem, Christopher Williams, Clair Rose, Samirah Perally, Guy Caljon, Jan Van Den Abbeele, Katherine Wongtrakul-Kish, Richard A. Gardner, Daniel Spencer, Michael J. Lehane, Álvaro Acosta-Serrano10 μg G. morsitans salivary proteins (lanes 1 and 2) and egg albumin (lanes 3 and 4) were incubated overnight with (+) or without (-) Endo H. Samples were resolved on a 12% SDS-PAGE gel and Coomassie stained. There was a notable shift in migration in 4 bands (1–4) after deglycosylation. These bands were excised, trypsinised and identified by mass spectrometry. 1, 5’ Nucleotidase; 2, TSGF 2/Adenosine deaminase; 3, TSGF 1; 4, Tsal 1/2. Asterisk indicates Endo H.
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ESI-LC-MSglycan profilesinsect bloodfeedingTrypanosome infectionTrypanosoma bruceitsetse genes encodingtrypanosome infectionhybrid-type Nïve tsetseglycoproteins bindmannosylated glycoproteinstranscriptional downregulationtrypanosome transmissionHILIC-UHPLC-FLRDC-SIGN C-type lectinsC-type lectinsmetacyclic trypanosomesoverlay assaysABtsetse saliva glycoproteinsparasite transmissionpaucimannosidic NMan 3 GlcNAc 2mannose receptor
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