Elevated Notch signaling does not cause margin defects (Related to Fig 4).

(A-B) ap-lacZ expression in an R72 control (w¹¹¹⁸) disc (A) and an R72 brat¹/+ disc (B). Dashed yellow lines are drawn next to the DV boundary to highlight it. (C-D) Anti-Wg (green) and anti-cleaved Dcp1 (magenta, gray in C’ and D’) immunostaining in an R48 control (w¹¹¹⁸) disc (C, C’) and an R48 brat¹/+ disc (D, D’). (E-F) NRE-GFP expression in an R24 control (w¹¹¹⁸) disc (E) and an R24 UAS-Nintra/+ disc (F). (G) Frequency of margin defects seen in adult wings after disc regeneration for control (w¹¹¹⁸) (n = 84) and UAS-Nintra/+ (n = 357) wings, from five independent experiments. (H-I) NRE-GFP expression in an undamaged control (w¹¹¹⁸) disc (H) and an undamaged aph-1^D35/+ disc (I). NRE-GFP/+ and NRE-GFP/aph-1^D35 animals were raised at room temperature and dissected during third instar. (J) Quantification of GFP intensity in undamaged control (w¹¹¹⁸) (n = 15) and aph-1^D35/+ (n = 15) discs. *** p < 0.0006. (K-L) NRE-GFP expression in an R24 control (w¹¹¹⁸) disc (K) and an R24 aph-1^D35/+ disc (L). (M) Quantification of GFP intensity in R24 control (w¹¹¹⁸) (n = 13) and R24 aph-1^D35/+ (n = 11) discs. * p < 0.02. (N) Frequency of margin defects in adult wings after disc regeneration for control (w¹¹¹⁸) (n = 21), brat¹/+ (n = 137), aph-1^D35/+ (n = 38) and brat¹/aph-1^D35(n = 80) wings. Error bars represent SEM. Student’s T-test used for statistical analyses. Scale bars are 100 μm.

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