Effects of the CdtB subunit of Helicobacter hepaticus on DNA damage and repair.

Images of 3-μm-tissue sections of Hep3B-derived mice engrafted tumors sequentially stained for 53BP1 (green) during a first labeling round and γH2AX (red) during a second labeling round, along with a counterstaining with DAPI (blue). Images 53BP-1/DAPI were captured using the microscope (wide field) that records the coordinates of each image, which allows repositioning on the same area after the γH2AX/DAPI immunodetection. Magnifications of selected areas are shown in boxes. Arrows indicates the overlapping of 53BP1 and γH2AX foci. The slight difference between the fields showing the 53BP1 and γH2AX labeling is due to the sequential repositioning between the sequential labeling. Abbreviations: CdtB, CdtB of H. hepaticus strain 3B1; DAPI, 4′, 6′-diamidino-2-phenylindol; H265L, H. hepaticus CdtB with the mutation His→Leu at residue 265 involved in catalytic activity.

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