Effects of reduced Brat on Wg and Myc expression are regeneration specific (Related to Fig 3).

(A-B) Anti-Wg immunostaining in an undamaged control (w¹¹¹⁸) disc (A) and an undamaged brat¹/+ disc (B). (C-D) Anti-Myc immunostaining in an undamaged control (w¹¹¹⁸) disc (C) and an undamaged brat¹/+ disc (D). (E) Quantification of Myc fluorescence intensity in undamaged control (w¹¹¹⁸) (n = 10) and brat¹/+ (n = 10) discs. Area for fluorescence intensity measurement was defined by wing pouch morphology and the elevated Myc expression domain in the wing pouch. Error bars represent SEM. (F) Representative adult wing after expression of UAS-wg during regeneration. (G) Representative adult wing after expression of UAS-ilp8 during regeneration. Student’s T-test used for statistical analyses. Scale bars are 100 μm for imaginal discs and 500 μm for adult wings.

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