Effects of PCC0208025 on IFN-γ secreted by CD3⁺ cells in vitro.

Human CD3⁺ cells were plated in the 96-well plate with a density of 1 × 10⁵ cells/well in 100 μL of DMEM containing 10% FBS. The 50 μL of aCD3 and aCD28 (final concentration of 1 μg/mL for each) were added into 96-well plate without or with 50 μL of human PD-L1 protein (final concentation of 10 nM). Then 50 μL of BMS-936559 or PCC0208025 solution with the final concentrations of 0.01, 0.1, 1 and 10 μM were added to the wells and cultured for 72 h. The supernatants were collected for detection of IFN-γ by using human IFN-γ ELISA Kit.

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