Effect of predicted mutations on binding of P4A2 mAb.

Parray, Hilal Ahmad; Narayanan, Naveen; Garg, Sonal; Rizvi, Zaigham Abbas; Shrivastava, Tripti; Kushwaha, Sachin; Singh, Janmejay; Murugavelu, Praveenkumar; Anantharaj, Anbalagan; Mehdi, Farha; Raj, Nisha; Singh, Shivam; Dandotiya, Jyotsna; Lukose, Asha; Jamwal, Deepti; Kumar, Sandeep; Chiranjivi, Adarsh K.; Dhyani, Samridhi; Mishra, Nitesh; Kumar, Sanjeev; Jakhar, Kamini; Sonar, Sudipta; Panchal, Anil Kumar; Tripathy, Manas Ranjan; Chowdhury, Shirlie Roy; Ahmed, Shubbir; Samal, Sweety; Mani, Shailendra; Bhattacharyya, Sankar; Das, Supratik; Sinha, Subrata; Luthra, Kalpana; Batra, Gaurav; Sehgal, Devinder; Medigeshi, Guruprasad R.; Sharma, Chandresh; Awasthi, Amit; Garg, Pramod Kumar; Nair, Deepak T.; Kumar, Rajesh

doi:10.1371/journal.ppat.1010994.s009

ppat.1010994.s009.pdf (471.5 kB)

Effect of predicted mutations on binding of P4A2 mAb.

journal contribution

posted on 2022-12-12, 18:39 authored by Hilal Ahmad Parray, Naveen Narayanan, Sonal Garg, Zaigham Abbas Rizvi, Tripti Shrivastava, Sachin Kushwaha, Janmejay Singh, Praveenkumar Murugavelu, Anbalagan Anantharaj, Farha Mehdi, Nisha Raj, Shivam Singh, Jyotsna Dandotiya, Asha Lukose, Deepti Jamwal, Sandeep Kumar, Adarsh K. Chiranjivi, Samridhi Dhyani, Nitesh Mishra, Sanjeev Kumar, Kamini Jakhar, Sudipta Sonar, Anil Kumar Panchal, Manas Ranjan Tripathy, Shirlie Roy Chowdhury, Shubbir Ahmed, Sweety Samal, Shailendra Mani, Sankar Bhattacharyya, Supratik Das, Subrata Sinha, Kalpana Luthra, Gaurav Batra, Devinder Sehgal, Guruprasad R. Medigeshi, Chandresh Sharma, Amit Awasthi, Pramod Kumar Garg, Deepak T. Nair, Rajesh Kumar

(A) Amino acid sequence of the variable regions of heavy and light chain of mAb P4A2 are denoted as P4A2-H and P4A2-L, respectively. Framework regions are shown in black, whereas the complementary determining region 1, 2 and 3 are highlighted in red, blue and green, respectively. (B) The mutations predicted by Maher et al. (2022) [5] are displayed in stick representation and coloured according to element. The spike-RBD, heavy and light chains of P4A2 Fab are shown in magenta, orange and cyan, respectively. None of the predicted mutations of the spike-RBD overlap with the residues that interact with P4A2 Fab and therefore it is possible that these mutations may not reduce the ability of P4A2 to neutralize the corresponding new variants of SARS-CoV-2.

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Effect of predicted mutations on binding of P4A2 mAb.

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