pone.0256701.s005.docx (16.05 kB)
Effect of melatonin addition to the IVF protocol on freezing/thawed sperm motion functionality assessed through FACS after 30-min, 120-min, and 180-min of the incubation period.
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posted on 2021-09-02, 17:31 authored by Juan Carlos Gutiérrez-Añez, Heiko Henning, Andrea Lucas-Hahn, Ulrich Baulain, Patrick Aldag, Birgit Sieg, Vivian Hensel, Doris Herrmann, Heiner Niemann(DOCX)
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sup > 8722plain tyrode ’many biological systemsflow cytometry dataaverage motility parameters>- derived blastocyststhaw sperm qualitysperm subpopulation structureassisted sperm analysisacrosome intact spermembryo development comparedearly embryo development180 min incubationshowed higher (<enhanced embryonic development11 supthawed bovine spermatozoamelatonin improves ratediv >< pembryo developmentenhanced (<30 min120 minvitro reduced (<p mapk13 greater (<viable spermrelative abundancepresent studypreparation protocolpotent nonoxidative stressmatured cumulusmainly dueincubated eitherhierarchical clusterfrequently reducedfertilizing abilityfemale gametesenzymatic antioxidantcontrol groupcontrol ).control ),complexes fertilizedadding melatoninactive mitochondria
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