pbio.3002415.s006.pdf (928.42 kB)
ELISA titrations of antibodies on HA-coated plates.
journal contribution
posted on 2023-12-21, 18:26 authored by Holly C. Simmons, Akiko Watanabe, Thomas H. Oguin III, Elizabeth S. Van Itallie, Kevin J. Wiehe, Gregory D. Sempowski, Masayuki Kuraoka, Garnett Kelsoe, Kevin R. McCarthyThe broadly binding, head interface directed S5V2-29 [22] was used as a positive control and an influenza B specific, RBS directed antibody, CR8033 [25], as a negative control for influenza A isolates. Data points represent the average of 3 technical replicates. The standard error of the mean is shown for each point. KDs were calculated from the curves fit to these data points. Figure data are in S2 Data.
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influenza virus hemagglutininmany potent antibodiesgenetically unrestricted antibodiesreceptor binding siteh3 isolates suggestnew h1 specificityh3 cross neutralizationh1 ha rbsresults suggesth3 exposureh3 crosssubtype neutralizationgroup neutralizationhistorical h1h1 haselected membersrbs mutationsrbs ).positions 133new classlater exposureaccepted correlate
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