ppat.1011815.s003.docx (24.55 kB)
EIA optical densities of brain regions of sheep infected with the WTD scrapie agent.
journal contribution
posted on 2023-12-04, 18:34 authored by Robyn D. Kokemuller, S. Jo Moore, Jifeng Bian, M. Heather West Greenlee, Justin J. GreenleeSamples from the brainstem at the level of the obex, cerebellum, and neocortex (10% homogenate at 1:1) of the animals tested for PrPSc immunoreactivity in Fig 2 were quantified using EIA (BSE-Scrapie Antigen Test Kit, EIA, IDEXX, Westbrook, ME) in increasing dilutions to determine the relative amount of PrPSc present in each brain region. The negative sample cut-off value of 0.201 was determined by adding 0.180 to the negative control sample provided in the kit.
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represents one examplenew strain propertiesnecropsied upon development72 months postprion agents occurmisfolded prion proteinshorter incubation periodsfastest incubation occurringsimilar incubation periodssc immunohistochemistry demonstrated prpdiv >< pinfluence disease phenotypewtd scrapie ).classical sheep scrapie13 – 7vrq sheep ).wtd scrapiedisease phenotypeinoculation ).rapid incubationfaster incubationsimilar patternsscrapie agentprion strainsphenotype switchphenotype changevrq sheep10 sheep124 scrapiewestern blotovinized miceoronasally challengedmultiple linesmouse bioassaymarkedly differentinterspecies transmissionevidence suggestenzyme immunoassayconformational stabilityclinical signsbrain tissuesattack rate7 inoculum
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