figshare
Browse
ppat.1011815.s002.docx (14.85 kB)

EIA data for mouse bioassay inocula.

Download (14.85 kB)
journal contribution
posted on 2023-12-04, 18:34 authored by Robyn D. Kokemuller, S. Jo Moore, Jifeng Bian, M. Heather West Greenlee, Justin J. Greenlee

Sheep inoculated with No.13-7, WTD scrapie sheep 2 (VRQ/VRQ sheep inoculated with deer cerebrum), x124, and WTD scrapie sheep 6 (VRQ/VRQ sheep inoculated with deer obex) used as inoculum source (1% homogenate at 1:1) for mouse bioassay were tested via EIA (BSE-Scrapie Antigen Test Kit, EIA, IDEXX, Westbrook, ME) in increasing dilutions to determine the amount of PrPSc.

(DOCX)

History

Usage metrics

    PLOS Pathogens

    Categories

    Keywords

    represents one examplenew strain propertiesnecropsied upon development72 months postprion agents occurmisfolded prion proteinshorter incubation periodsfastest incubation occurringsimilar incubation periodssc immunohistochemistry demonstrated prpdiv >< pinfluence disease phenotypewtd scrapie ).classical sheep scrapie13 – 7vrq sheep ).wtd scrapiedisease phenotypeinoculation ).rapid incubationfaster incubationsimilar patternsscrapie agentprion strainsphenotype switchphenotype changevrq sheep10 sheep124 scrapiewestern blotovinized miceoronasally challengedmultiple linesmouse bioassaymarkedly differentinterspecies transmissionevidence suggestenzyme immunoassayconformational stabilityclinical signsbrain tissuesattack rate7 inoculum

    Licence

    CC BY 4.0

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC