posted on 2021-06-23, 16:33authored byXiaoya Liu, Xicheng Zhang, Junyan Tang, Yang Meng, Liping Zhao, Wenjing Shi, Feifei Tao, Zigui Kan, Fei Wang, Caolong Li
Circulating
tumor cells (CTCs) play a pivotal role in the early
diagnosis of pheochromocytoma (PCC). Herein, we fabricated a new dual-targeting
nanoprobe for coinstantaneous identification of rare PCC-CTCs from
peripheral blood via targeting the norepinephrine transporter (NET)
and somatostatin receptor SSTR2 overexpressed on the surface of PCC
cells. Meta-iodobenzylguanidine (MIBG) functionalized magnetic Fe3O4 and octreotide (DOTA) decorated signal amplification
Ag@SiO2 nanosphere were used to capture and detect PCC-CTCs
by binding to NET and SSTR2. The proposed dual-targeting sensor achieved
good reproducibility and high sensitivity for the monitoring of PC12
in the concentration range from 5 to 5 × 104 cells
mL–1, with detection limits of 2 cell/mL. This strategy
opens a new approach for simple, sensitive, and rapid determination
of PCC biomarkers, which shows great potential in early diagnosis,
prognosis, and therapeutic evaluation of PCC.