posted on 2021-09-01, 16:39authored byYue Hu, Liang Zhu, Xuecui Mei, Jinsen Liu, Zhongping Yao, Yingchun Li
Development
of sensors uniting different sensing principles is
in line with the concept of reliable, comprehensive, and diversified
equipment construction. However, the current exploration in this field
is obstructed by compromise of reaction conditions and inevitable
mutual interference arising from different sensing modes. This work
reported a closed bipolar electrode (c-BPE) strategy for dual-modality
detection or dual-target detection. To this end, a c-BPE sensing platform
installed in physically separated anode and cathode compartments was
well designed and carefully optimized. If luminol was present in the
anode section and Prussian blue (PB) was at the cathode part, single
stimulation could realize electrochemiluminescence (ECL) from luminol
at the anode and conversion of PB to Prussian white (PW) at the cathode.
The latter reaction helped elevate the ECL signal and also prepared
for colorimetric detection as color change from PW to PB under the
trigger of oxidant (like H2O2) was used to track
the content of the oxidant. Thus, dual signals were obtained for dual-modality
detection of single target or the detection of different targets was
realized at different poles. Detection of glucose was carried out
to validate the application for dual-modality detection, while VLDL/AChE
and NADH/H2O2 assays illustrated the potential
of dual-target detection. The proposed platform possesses outstanding
sensing performance including selectivity, repeatability, long-term
stability, accuracy, and so forth. This work implements a breakthrough
in designing dual-mode sensors and is expected to present a rational
basis for development of a diversified sensing platform.