posted on 2021-12-27, 14:41authored byHaiyue Peng, Ting Wang, Guorui Li, Jing Huang, Quan Yuan
Activity-based near-infrared (NIR)
fluorescent probes provide powerful
tools for diagnosis of diseases. However, most of these probes suffer
from low specificity due to “off-target” reaction. The
dual-locked strategy, which utilizes two biomarkers as triggers, can
increase the specificity and precision of diagnosis. Here, we report
a dual-locked NIR probe, MB-m-borate, which releases fluorophore methylene blue (MB) after
hydrogen peroxide–tyrosinase (H2O2-TYR)
cascade activation. Both MB-m-borate and its intermediate MB-m-phenol (the
product after H2O2 activation) show almost nondetectable
fluorescence. MB-m-borate exhibits “turn
on” fluorescence upon H2O2-TYR cascade
activation. The further live cell bioimaging results indicate that MB-m-borate only responds to melanoma cells,
providing it as a robust probe for precise detection of melanoma.
Finally, the probe is applied for the diagnosis of melanoma in vivo
with a xenogeneic mouse model.