Gating strategy.

Cimini, Eleonora; Viola, Domenico; Cabeza-Cabrerizo, Mar; Romanelli, Antonella; Tumino, Nicola; Sacchi, Alessandra; Bordoni, Veronica; Casetti, Rita; Turchi, Federica; Martini, Federico; Bore, Joseph A.; Raymond Koundouno, Fara; Duraffour, Sophie; Michel, Janine; Holm, Tobias; Zekeng, Elsa Gayle; Cowley, Lauren; Dorival, Isabel Garcia; Doerrbecker, Juliane; Hetzelt, Nicole; Baum, Jonathan H. J.; Portmann, Jasmine; Wölfel, Roman; Gabriel, Martin; Miranda, Osvaldo; Díaz, Graciliano; Díaz, José E.; Fleites, Yoel A.; Piñeiro, Carlos A.; Castro, Carlos M.; Koivogui, Lamine; Magassouba, N’Faly; Diallo, Boubacar; Ruibal, Paula; Oestereich, Lisa; Wozniak, David M.; Lüdtke, Anja; Becker-Ziaja, Beate; R. Capobianchi, Maria; Ippolito, Giuseppe; W. Carroll, Miles; Günther, Stephan; Di Caro, Antonino; Muñoz-Fontela, César; Agrati, Chiara

doi:10.1371/journal.pntd.0005645.s001

pntd.0005645.s001.pdf (219.46 kB)

Gating strategy.

journal contribution

posted on 2017-05-30, 17:31 authored by Eleonora Cimini, Domenico Viola, Mar Cabeza-Cabrerizo, Antonella Romanelli, Nicola Tumino, Alessandra Sacchi, Veronica Bordoni, Rita Casetti, Federica Turchi, Federico Martini, Joseph A. Bore, Fara Raymond Koundouno, Sophie Duraffour, Janine Michel, Tobias Holm, Elsa Gayle Zekeng, Lauren Cowley, Isabel Garcia Dorival, Juliane Doerrbecker, Nicole Hetzelt, Jonathan H. J. Baum, Jasmine Portmann, Roman Wölfel, Martin Gabriel, Osvaldo Miranda, Graciliano Díaz, José E. Díaz, Yoel A. Fleites, Carlos A. Piñeiro, Carlos M. Castro, Lamine Koivogui, N’Faly Magassouba, Boubacar Diallo, Paula Ruibal, Lisa Oestereich, David M. Wozniak, Anja Lüdtke, Beate Becker-Ziaja, Maria R. Capobianchi, Giuseppe Ippolito, Miles W. Carroll, Stephan Günther, Antonino Di Caro, César Muñoz-Fontela, Chiara Agrati

The following gates were used for data analysis: -G1: Lymphocyte gate (Panel A); -G2:CD3+ cells; G3: CD3-cells (Panel B); -G4: Vδ2+ among G2 (Panel D); -G5: CD56+CD16-NK cells among G3 (bright); G6: CD56+CD16+ NK cells among G3 (Dim), G7: CD56-CD16+ NK cells among G3 (Neg) (Panel C). -The expression of CCR7, CD95 and CTLA4 was analyzed in G4 (Panel E); -The expression of CD69, NK p46 and CD158a was evaluated in G6 and G7 (Panel F-G).

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